منابع مشابه
revolution of dna sequencing method from the past until today
dna sequence determination is a tremendous human achievement. dna sequencing includes several methods and technologies in use for determining the order of the nucleotide bases (adenine, guanine, cytosine, and thymine) in a molecule of dna. knowledge of dna sequences has become indispensable for basic biological research, other research branches utilizing dna sequencing, and in numerous applied ...
متن کاملTranscriptional sequencing: A method for DNA sequencing using RNA polymerase.
We have developed a sequencing method based on the RNA polymerase chain termination reaction with rhodamine dye attached to 3'-deoxynucleoside triphosphate (3'-dNTP). This method enables us to conduct a rapid isothermal sequencing reaction in <30 min, to reduce the amount of template required, and to do PCR direct sequencing without the elimination of primers and 2'-dNTP, which disturbs the San...
متن کاملSequencing Design DNA: a Set of Methodological Artifacts for sequencing Socio-Technical Design Routines
With the introduction of new digital and physical tools into the workplace, the process of design has dramatically changed over the past few decades. Thus, design processes have evolved into many forms which vary, not only between organizations, but within organizations, and even within teams over time. These myriad “mutations” of the design process call for a new method to identify patterns of...
متن کاملFast, manual, nonradioactive method for DNA sequencing.
We describe a protocol that allows nonradioactive detection of sequencing products after manual, direct, solid-phase sequencing of polymerase chain reaction-amplified DNA. The amplified DNA fragment to be studied is biotinylated at the 5' end of one of the two oligonucleotide primers used for amplification, allowing coupling to streptavidin-coated magnetic beads. The immobilized double-stranded...
متن کاملAn iterative and regenerative method for DNA sequencing.
This paper presents, to our knowledge, the first iterative DNA sequencing method that regenerates the product of interest during each iterative cycle, allowing it to overcome the critical obstacles that impede alternative iterative approaches to DNA sequencing: loss of product and the accumulation of background signal due to incomplete reactions. It can sequence numerous double-stranded (ds) DN...
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ژورنال
عنوان ژورنال: Japanese Journal of Thrombosis and Hemostasis
سال: 1998
ISSN: 1880-8808,0915-7441
DOI: 10.2491/jjsth.9.190